Detection of hepatitis B virus in serum using amplification of viral DNA by means of the polymerase chain reaction.
作者： Ryo Sumazaki , Manfred Motz , Hans Wolf , Jutta Heinig , Wolfgang Jilg
关键词:
摘要: A new assay was developed for the detection of hepatitis B virus (HBV) in human serum using amplification a short viral DNA sequence by means polymerase chain reaction. As little as 0.4 fg DNA, corresponding to about 130 genome equivalents, per ml could be detected after procedure. This number patients with proven or suspected chronic HBV infection who were all negative conventional hybridisation assay. We found six HBeAg-positive and three eight HBeAg-negative HBsAg carriers, well 11 liver disease antibodies against core antigen (anti-HBc) sole marker infection, five apparently healthy individuals showing only anti HBc. Thus, this method is an important improvement diagnosis persistent infections, especially where definitive serological not possible.
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