Functional characterizations of β-glucosidases involved in aroma compound formation in tea (Camellia sinensis).
作者： Ying Zhou , Lanting Zeng , Jiadong Gui , Yinyin Liao , Jianlong Li
DOI: 10.1016/J.FOODRES.2017.03.049
关键词:
摘要: Tea (Camellia sinensis) aroma is an important factor affecting tea quality. Many compounds are present as glycosidically conjugated forms in leaves, and can be hydrolyzed by β-glucosidase (β-Glu) β-primeverosidase to release free aromas. β-Primeverosidase has been identified functionally characterized, while β-Glu not leaves. In the study, we established a yeast expression system recombine CsGH1BG1, CsGH3BG1, CsGH5BG1, which belonged GH1, GH3, GH5 families plants, respectively. These three recombinant Csβ-Glus β-glucopyranosidically aromas form aromas, suggesting that there was no specific for hydrolysis of vitro. Furthermore, subcellular localization indicated CsGH1BG1 CsGH3BG1 were located cytosol vacuole, respectively, CsGH5BG1 cell wall. This suggested might responsible leaves during manufacturing process. study provides first evidence will advance understanding formation.
sci-hub.se 参考文章(38)
Peter Winterhalter, George K. Skouroumounis, Glycoconjugated aroma compounds: Occurrence, role and biotechnological transformation Advances in Biochemical Engineering/Biotechnology. ,vol. 55, pp. 73- 105 ,(1997) , 10.1007/BFB0102063
Aysun Şener, Extraction, partial purification and determination of some biochemical properties of β–glucosidase from Tea Leaves (Camellia sinensis L.) Journal of Food Science and Technology-mysore. ,vol. 52, pp. 8322- 8328 ,(2015) , 10.1007/S13197-015-1915-Z
Jiadong Gui, Xiumin Fu, Ying Zhou, Tsuyoshi Katsuno, Xin Mei, Rufang Deng, Xinlan Xu, Linyun Zhang, Fang Dong, Naoharu Watanabe, Ziyin Yang, Does Enzymatic Hydrolysis of Glycosidically Bound Volatile Compounds Really Contribute to the Formation of Volatile Compounds During the Oolong Tea Manufacturing Process Journal of Agricultural and Food Chemistry. ,vol. 63, pp. 6905- 6914 ,(2015) , 10.1021/ACS.JAFC.5B02741
Sue M Cutfield, Gideon J Davies, Garib Murshudov, Bryan F Anderson, Peter C.E Moody, Patrick A Sullivan, John F Cutfield, The structure of the exo-beta-(1,3)-glucanase from Candida albicans in native and bound forms: relationship between a pocket and groove in family 5 glycosyl hydrolases. Journal of Molecular Biology. ,vol. 294, pp. 771- 783 ,(1999) , 10.1006/JMBI.1999.3287
Barbara Reinhold-Hurek, Tamara Maes, Sabrina Gemmer, Marc Van Montagu, Thomas Hurek, An Endoglucanase Is Involved in Infection of Rice Roots by the Not-Cellulose-Metabolizing Endophyte Azoarcus Sp. Strain BH72 Molecular Plant-microbe Interactions. ,vol. 19, pp. 181- 188 ,(2006) , 10.1094/MPMI-19-0181
Masaharu Mizutani, Hidemitsu Nakanishi, Jun-ichi Ema, Seung-Jin Ma, Etsuko Noguchi, Misa Inohara-Ochiai, Masako Fukuchi-Mizutani, Masahiro Nakao, Kanzo Sakata, Cloning of β-Primeverosidase from Tea Leaves, a Key Enzyme in Tea Aroma Formation Plant Physiology. ,vol. 130, pp. 2164- 2176 ,(2002) , 10.1104/PP.102.011023
Kanako Kudo, Akira Watanabe, Seiryu Ujiie, Takahiro Shintani, Katsuya Gomi, Purification and enzymatic characterization of secretory glycoside hydrolase family 3 (GH3) aryl β-glucosidases screened from Aspergillus oryzae genome Journal of Bioscience and Bioengineering. ,vol. 120, pp. 614- 623 ,(2015) , 10.1016/J.JBIOSC.2015.03.019
Frauke V. Härtel, Anders Brandt, Characterization of a Brassica napus Myrosinase Expressed and Secreted by Pichia pastoris Protein Expression and Purification. ,vol. 24, pp. 221- 226 ,(2002) , 10.1006/PREP.2001.1562
Cheng-Fang Tsai, Xiao Qiu, Jin-Hao Liu, A comparative analysis of two cDNA clones of the cellulase gene family from anaerobic fungus Piromyces rhizinflata Anaerobe. ,vol. 9, pp. 131- 140 ,(2003) , 10.1016/S1075-9964(03)00087-8
Z. Lewis Liu, Scott A. Weber, Michael A. Cotta, Shi-Zhong Li, A new β-glucosidase producing yeast for lower-cost cellulosic ethanol production from xylose-extracted corncob residues by simultaneous saccharification and fermentation. Bioresource Technology. ,vol. 104, pp. 410- 416 ,(2012) , 10.1016/J.BIORTECH.2011.10.099