Interleukin-15 Dendritic Cells Harness NK Cell Cytotoxic Effector Function in a Contact- and IL-15-Dependent Manner

摘要: The contribution of natural killer (NK) cells to the treatment efficacy dendritic cell (DC)-based cancer vaccines is being increasingly recognized. Much current efforts optimize this form immunotherapy are therefore geared towards harnessing NK cell-stimulatory ability DCs. In study, we investigated whether generation human monocyte-derived DCs with interleukin (IL)-15 followed by activation a Toll-like receptor stimulus endows these DCs, commonly referred as “IL-15 DCs”, capacity stimulate cells. head-to-head comparison “IL-4 DCs” used routinely for clinical studies, IL-15 were found induce more activated, cytotoxic effector phenotype in cells, particular CD56bright subset. With exception GM-CSF, no significant enhancement cytokine/chemokine secretion was observed following co-culture but not IL-4 promoted tumoricidal activity both NK-sensitive and NK-resistant targets. This effect require cell-to-cell contact be mediated DC surface-bound IL-15. study shows that can express membrane-bound through which they enhance function. lack on “gold-standard” their consequent inability effectively promote cytotoxicity may have important implications future design DC-based vaccine studies.