RNA interference shows interactions between mouse brainstem angiotensin AT1 receptors and angiotensin‐converting enzyme 2
作者： Zhanyi Lin , Yanfang Chen , Wenfeng Zhang , Alex F. Chen , Shuguang Lin
DOI: 10.1113/EXPPHYSIOL.2007.041657
关键词:
摘要: Angiotensin (Ang) AT1 receptors and Ang-converting enzymes (ACE ACE2) are expressed in the dorsal vagal complex (DVC) of brainstem. The aim this study was to examine vivo interactions between brainstem Ang receptors, ACE ACE2 using small, hairpin RNA (shRNA) gene-silencing methods. takes advantage bilateral expression renin–angiotensin system (RAS) markers. Adenovirus vectors (Ad, 2.0 × 109 c.f.u. ml−1, 200 nl) carrying interference small for either AngAT1a (Ad-AT1a-shRNA) or AngAT1b (Ad-AT1b-shRNA) were microinjected into right side DVC. Ad-LacZ control injected left side. Brainstems processed with situ hybridization immunochemistry. Results showed that: (1) Ad-AT1a-shRNA downregulated AT1a mRNA by 61.2 ± 6.8% (P < 0.01) Ad-AT1b-shRNA AT1b 51.6 5.2% 0.01); (2) downregulation associated decreased (decrease 29.0 14.5%, P 0.01), while reduction Ad-AT1b had no effect; (3) not altered (RNAi) treatment; (4) immunochemical staining agreement changes observed. These results demonstrate utility gene silencing functional specificity. Both induced site- subtype-specific receptor expression. Gene that there RAS regulatory enzyme, ACE2.
nova.edu
core.ac.uk
physoc.org参考文章(51)
M C Chappell, K B Brosnihan, D I Diz, C M Ferrario, Identification of angiotensin-(1-7) in rat brain. Evidence for differential processing of angiotensin peptides. Journal of Biological Chemistry. ,vol. 264, pp. 16518- 16523 ,(1989) , 10.1016/S0021-9258(19)84737-3
J. M. Burson, G. Aguilera, K. W. Gross, C. D. Sigmund, Differential expression of angiotensin receptor 1A and 1B in mouse. American Journal of Physiology-endocrinology and Metabolism. ,vol. 267, ,(1994) , 10.1152/AJPENDO.1994.267.2.E260
Yanfang Chen, Yiwen Liu-Stratton, Hamdy Hassanain, David R. Cool, Mariana Morris, Dietary sodium regulates angiotensin AT1a and AT1b mRNA expression in mouse brain. Experimental Neurology. ,vol. 188, pp. 238- 245 ,(2004) , 10.1016/J.EXPNEUROL.2004.04.005
Maria José Alves Rocha, Yanfang Chen, Gabriela Ravanelli Oliveira, Mariana Morris, Physiological regulation of brain angiotensin receptor mRNA in AT1a deficient mice. Experimental Neurology. ,vol. 195, pp. 229- 235 ,(2005) , 10.1016/J.EXPNEUROL.2005.05.011
V. A. Alvarez, D. A. Ridenour, B. L. Sabatini, Retraction of synapses and dendritic spines induced by off-target effects of RNA interference. The Journal of Neuroscience. ,vol. 26, pp. 7820- 7825 ,(2006) , 10.1523/JNEUROSCI.1957-06.2006
Lenice K Becker, Gisele M Etelvino, Thomas Walther, Robson AS Santos, Maria J Campagnole-Santos, None, Immunofluorescence localization of the receptor Mas in cardiovascular-related areas of the rat brain American Journal of Physiology-heart and Circulatory Physiology. ,vol. 293, ,(2007) , 10.1152/AJPHEART.00141.2007
Sham S. Kakar, Kristen K. Riel, Jimmy D. Neill, Differential expression of angiotensin II receptor subtype mRNAs (AT-1A and AT-1B) in the brain Biochemical and Biophysical Research Communications. ,vol. 185, pp. 688- 692 ,(1992) , 10.1016/0006-291X(92)91680-O
C.H. Block, R.A.S. Santos, K.B. Brosnihan, C.M. Ferrario, Immunocytochemical localization of angiotensin-(1–7) in the rat forebrain Peptides. ,vol. 9, pp. 1395- 1401 ,(1988) , 10.1016/0196-9781(88)90208-2
Theodora Alexiou, Wee Ming Boon, Derek A Denton, Robert Di Nicolantonio, Lesley L Walker, Michael J McKinley, Duncan J Campbell, Angiotensinogen and angiotensin-converting enzyme gene copy number and angiotensin and bradykinin peptide levels in mice. Journal of Hypertension. ,vol. 23, pp. 945- 954 ,(2005) , 10.1097/01.HJH.0000166834.32817.41
Olaf Jöhren, Tadashi Inagami, Juan M. Saavedra, AT1A, AT1B, and AT2 angiotensin II receptor subtype gene expression in rat brain. Neuroreport. ,vol. 6, pp. 2549- 2552 ,(1995) , 10.1097/00001756-199512150-00024