Rat liver ischemia-reperfusion-induced apoptosis and necrosis are decreased by FK506 pretreatment.
作者： Dominique Crenesse , Marina Laurens , Catherine Heurteaux , Raffaele Cursio , Marie Christine Saint-Paul
DOI: 10.1016/S0014-2999(03)01977-0
关键词:
摘要: The aim of this study was to demonstrate that tacrolimus (FK506) has a hepatoprotective effect by reducing ischemia-reperfusion-induced apoptosis and necrosis, both which lead post-surgical liver dysfunction. An ischemia-reperfusion model primary cultured rat hepatocytes subjected hypoxic reoxygenation phases, mimicking the surgical process, were used. c-Jun N-terminal kinase 1/stress-activated protein 1 (JNK1/SAPK1) activation leads caspase 3 activation, trigger apoptosis. status JNK1/SAPK1 evaluated immunoprecipitation or Western-blotting experiments. Apoptosis assessed measuring TUNEL (terminal deoxynucleotidyltransferase-mediated deoxyuridine triphosphate-biotin nick-end labeling) reaction. Necrosis histologically. Tacrolimus improved survival rate rats ischemia-reperfusion. After FK506 pretreatment, necrosis reduced, significantly decreased. In hypoxia-reoxygenation-subjected hepatocytes, reduced activation. liver, prevented necrosis.
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