Long noncoding RNAs are spatially correlated with transcription factors and regulate lung development.

摘要: Long noncoding RNAs (lncRNAs) are thought to play important roles in regulating gene transcription, but few have well-defined expression patterns or known biological functions during mammalian development. Using a conservative pipeline identify lncRNAs that functions, we identified 363 the lung and foregut endoderm. Importantly, show these spatially correlated with transcription factors across genome. In-depth analyses of genomic loci adjacent critical Nkx2.1, Gata6, Foxa2 (forkhead box a2), Foxf1 mimic their protein-coding neighbor. Loss-of-function analysis demonstrates two lncRNAs, LL18/NANCI (Nkx2.1-associated intergenic RNA) LL34, distinct endoderm development by controlling developmental pathways, including retinoic acid signaling. In particular, acts upstream Nkx2.1 downstream from Wnt signaling regulate expression. These studies reveal an role multiple aspects often through regulation factor