Site-specific binding of polymerase-containing particles of the Giardia lamblia double-stranded RNA virus to the viral plus-strand RNA

摘要: The non-segmented, double-stranded RNA genome of the Giardia lamblia virus (GLV) contains two genes encoding major capsid protein (gag) and a fusion gag with viral RNA-dependent polymerase (pol). Computer analysis revealed three putative stem-loop structures that were predicted to mediate replication, transcription packaging GLV genomic by binding pol domain virus-encoded protein. To provide evidence these postulated RNA/protein interactions, gel retardation assays employed examine potential capacity various genome-related sequences native protein(s). Viral proteins obtained disrupting purified particles under low-ionic-strength conditions. resulting maintained their activity in presence thus appeared be suitable tools for analyses GLV-protein-mediated reactions. A 72-nt short single-stranded vitro transcript containing structure participate (+)-strand bound specifically disrupted particles. RNAs modified motifs this failed bind capsid.