Metabolomic Analysis Using Liquid Chromatography/Mass Spectrometry for Gastric Cancer

摘要: Metabolomics is a post-genomics research field for analysis of low molecular weight compounds in biological samples and has shown great potentials elucidating complex mechanisms associated with diseases. However, metabolomics studies on gastric cancer (GC), which the second leading cause death worldwide, remain scarce, to phenotypes are also still not fully understood. This study reports that metabolic pathways can be exploited as biomarkers diagnosis treatment GC progression case study. Importantly, urinary metabolites patterns were analyzed by high-throughput liquid chromatography mass spectrometry (LC-MS) strategy coupled chemometric evaluation. Sixteen (nine upregulated seven downregulated) differentially expressed may thus serve potential human GC. These mainly involved multiple pathways, including citrate cycle (malic acid, succinic 2-oxoglutarate, citric acid), cyanoamino acid metabolism (glycine, alanine), primary bile biosynthesis taurine, glycocholic arginine proline (urea, l-proline), fatty (hexadecanoic among others. Network validated close association between these identified altered variety processes. results suggest urine profiles have detecting aid understanding its underlying mechanisms. It provides insight into disease pathophysiology basis developing therapeutic interventions