Metabolomic investigation of gastric cancer tissue using gas chromatography/mass spectrometry.
作者： Hao Wu , Ruyi Xue , Zhaoqing Tang , Chunhui Deng , Taotao Liu
DOI: 10.1007/S00216-009-3317-4
关键词: Gas chromatography–mass spectrometry 、 Metabolite 、 Metabolomics 、 Pathology 、 Cancer 、 Chemistry 、 Area under the curve 、 Analytical chemistry 、 Gastric mucosa 、 Biomarker (medicine) 、 Biopsy
摘要: Gastric cancer screening or diagnosis is mainly based on endoscopy and biopsy. The aim of this study was to identify the difference metabolomic profile between normal malignant gastric tissue, further explore tumor biomarkers. Chemical derivatization together with gas chromatography/mass spectrometry (GC/MS) utilized obtain information non-malignant tissues mucosae in 18 patients. Acquired data analyzed using Wilcoxon rank sum test find tissue metabolic biomarkers for cancer. A diagnostic model constructed principal component analysis (PCA), assessed receiver-operating characteristic (ROC) curves. Results showed that metabolites were detected differently adjacent mucosa. Five also non-invasive tumors invasive tumors. could discriminate from an area under curve (AUC) value 0.9629, another clinical staging AUC 0.969. We conclude different normal, selected probably be applied
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