Effect of estradiol and progesterone on human endometrial aromatase activity in primary cell culture.

摘要: Isolated human endometrial stromal cells were cultured in RPMI 1640 medium containing insulin, antibiotics and 10% fetal calf serum. After the developed a confluent monolayer, estradiol (E2), progesterone (P), or both separately added to culture continuously for various periods of time. Aromatase activity was measured control hormone-treated by incubating intact with [3H]testosterone isolating estrogens at end incubation. E2 alone did not change aromatase activity. P caused an 8– >40-fold increase over values (1 7 fmol/mg protein·h), further increased presence (20- 100-fold). The stimulation found be time- dose-dependent blocked actinomycin D. Maximal reached after treated 300 nM 3 days. At 30 P, concentration similar plasma level during lutea...