Ultrasound-Assisted Rapid Extraction of Bacterial Intracellular Medium-Chain-Length Poly(3-Hydroxyalkanoates) (mcl-PHAs) in Medium Mixture of Solvent/Marginal Non-solvent

摘要: Intracellular medium-chain-length poly(3- hydroxyalkanoates) (PHAs) produced by Pseudomonasputida Bet001 were extracted in an ultrasound-assisted process. A mixture of acetone (solvent) and heptane (marginal non-solvent) was used as the extraction medium. The effects volumetric energy dissipation, medium ratio irradiation time on process investigated. Sonication frequency 37 kHz marginal non-solvent facilitated Following optimization, high PHA rate \({74 \times 10^{-3}{\rm g}}\) \({{\rm g}^{-1}}\) dried biomass min}^{-1}}\) observed at ultrasonic output \({1151 \pm 3 {\rm J ml}^{-1}}\) with 50:50 solvent/marginal for 5 min. showed good stability under rapid