Sequence specificity of the human immunodeficiency virus type 2 (hiv-2) long terminal repeat u3 region in vivo allows subtyping of the principal hiv-2 viral subtypes a and b.
作者： Neil Berry , Koya Ariyoshi , Peter Balfe , Richard Tedder , Hilton Whittle
DOI: 10.1089/088922201750063197
关键词:
摘要: Sequences from the nef/LTR overlap region of human immunodeficiency virus type 2 (HIV-2) genome were amplified uncultured peripheral blood mononuclear cells (PBMCs) 40 HIV-2-infected individuals in The Gambia, West Africa. Additional sequences plasma three donors also derived. Analysis HIV-2 U3 LTR transcription factor elements (PuB-1, p-ets, PuB-2, peri-κB, and NF-κB sites) indicated a relatively high level conservation vivo. immediately 3′ nef termination codon, which exhibits clade-dependent specificity, was targeted by PCR to differentiate subtype A B infections, two principal clinical subtypes. All samples analyzed (n = 43) Gambia identified as combination sequence analysis subtype-specific amplification subtypes B. Differential represents rapid means differentiating infec...
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