Columbamine suppresses the proliferation and neovascularization of metastatic osteosarcoma U2OS cells with low cytotoxicity

摘要: Osteosarcoma is one of the most common malignant bone tumors in children and adolescents. Although extensive efforts have been made anti-osteosarcoma therapy recent decades, there are no effective low-toxicity drugs for treating patients with metastatic osteosarcoma. Hence, potent anti-metastatic osteosarcoma highly desired. In this study, we explored novel small molecular agents found that columbamine (COL), an active component herb Coptis chinensis, inhibited proliferation neovascularization U2OS cells. COL effectively suppressed cell vitro IC(50) 21.31±0.38μM, low cytotoxicity. Mechanistic studies revealed induces cycle arrest at G2/M transition, which associated attenuating CDK6 gene expression diminishing STAT3 phosphorylation. did not significantly promote apoptosis any dosages tested. Additionally, cell-mediated neovascularization, was accompanied by down-regulation matrix metalloproteinase (MMP) 2 reduction migration, adhesion, invasion. Taken together, our data show exerts anti-proliferative anti-vasculogenic effects on human cells toxicity. These results warrant further investigation as a potential anti-cancer drug.