Characterization of a specific polyclonal antibody against 13-hydroperoxyoctadecadienoic acid-modified protein: formation of lipid hydroperoxide-modified apoB-100 in oxidized LDL.
作者： Y Kato , Y Makino , T Osawa
DOI: 10.1016/S0022-2275(20)37417-4
关键词:
摘要: Lipid hydroperoxide may react with protein or amino phospholipid without secondary decomposition. We prepared a polyclonal antibody to lipid hydroperoxide-modified proteins using 13S-hydroperoxy-9Z, 11E-octadecadienoic acid-modified keyhole limpet hemocyanin (13-HPODE-KLH) as immunogen. The recognized 13-HPODE-modified bovine serum albumin (BSA), but not aldehyde-modified proteins, such malondialdehyde-modified BSA. also adducts derived from 13-HPODE and 11E, 15Z-octadecatrienoic acid (13-HPOTRE(alpha)). oxidized alpha-linolenic acid- linoleate-protein were by the antibody. Oxidized phospholipid-protein scarcely However, when ester bonds of phospholipids containing linoleic hydrolyzed alkaline treatment, cross-reactivities appeared. result suggests that can directly indirectly, carboxyl terminal (COOH) in an adduct was needed epitope. LDL (ox-LDL) incubation copper ion 2,2'-azobis(2-amidinopropane)dihydrochloride (AAPH), formation apolipoprotein confirmed A slight immunoreactivity observed ox-LDL treatment. When treated alkali hydrolyze lipid, enhanced antigenicity appeared time-dependency. results suggest formed during oxidation LDL.
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