Purification of putative Ca2+ channel protein from rabbit skeletal muscle. Determination of the amino-terminal sequence.
作者： N Nakayama , T L Kirley , P L Vaghy , E McKenna , A Schwartz
DOI: 10.1016/S0021-9258(18)48279-8
关键词:
摘要: A putative Ca2+ channel protein was purified from rabbit skeletal muscle transverse tubules with the combined use of lectin affinity chromatography and ion-exchange chromatography, followed by sucrose density gradient centrifugation. The major component preparation detected sodium dodecyl sulfate-gel electrophoresis a 150 kDa when reduced 20 mM dithiothreitol 191-kDa treated N-ethylmaleimide. Therefore, this appears to be identical alpha subunit previously described (Curtis, B. M., Catterall, W. A. (1984) Biochemistry 23, 2113-2118). This preparative electrophoresis, electroelution and/or electroblotting, its amino acid composition NH2-terminal sequence were determined. is: NH2-Glu-Pro-Phe-Pro-Ser-Ala-Val-X-Ile-Lys-Ser-X-Val-X-Lys-Met-Gln-.
sci-hub.st 参考文章(30)
Joel VANDEKERCHOVE, Guy BAUW, Magda PUYPE, Jozef DAMME, Marc MONTAGU, Protein-blotting on Polybrene-coated glass-fiber sheets. A basis for acid hydrolysis and gas-phase sequencing of picomole quantities of protein previously separated on sodium dodecyl sulfate/polyacrylamide gel. FEBS Journal. ,vol. 152, pp. 9- 19 ,(1985) , 10.1111/J.1432-1033.1985.TB09157.X
C Hidalgo, M E Gonzalez, R Lagos, Characterization of the Ca2+- or Mg2+-ATPase of transverse tubule membranes isolated from rabbit skeletal muscle. Journal of Biological Chemistry. ,vol. 258, pp. 13937- 13945 ,(1983) , 10.1016/S0021-9258(17)44007-5
Y H Lau, A H Caswell, J P Brunschwig, Isolation of transverse tubules by fractionation of triad junctions of skeletal muscle. Journal of Biological Chemistry. ,vol. 252, pp. 5565- 5574 ,(1977) , 10.1016/S0021-9258(19)63388-0
Y.H. Lau, A.H. Caswell, J.P. Brunschwig, R.j. Baerwald, M. Garcia, Lipid analysis and freeze-fracture studies on isolated transverse tubules and sarcoplasmic reticulum subfractions of skeletal muscle. Journal of Biological Chemistry. ,vol. 254, pp. 540- 546 ,(1979) , 10.1016/S0021-9258(17)37949-8
J Barhanin, M Fosset, J P Galizzi, M Lazdunski, M Borsotto, Characterization and photoaffinity labeling of receptor sites for the Ca2+ channel inhibitors d-cis-diltiazem, (+/-)-bepridil, desmethoxyverapamil, and (+)-PN 200-110 in skeletal muscle transverse tubule membranes. Journal of Biological Chemistry. ,vol. 261, pp. 1393- 1397 ,(1986) , 10.1016/S0021-9258(17)36105-7
M Borsotto, J Barhanin, M Fosset, M Lazdunski, The 1,4-dihydropyridine receptor associated with the skeletal muscle voltage-dependent Ca2+ channel. Purification and subunit composition. Journal of Biological Chemistry. ,vol. 260, pp. 14255- 14263 ,(1985) , 10.1016/S0021-9258(17)38711-2
David B . Teplow, Ruedi H. Aebersold, Leroy E . Hood, Stephen B . H. Kent, Electroblotting onto activated glass. High efficiency preparation of proteins from analytical sodium dodecyl sulfate-polyacrylamide gels for direct sequence analysis Journal of Biological Chemistry. ,vol. 261, pp. 4229- 4238 ,(1986) , 10.1016/S0021-9258(17)35652-1
M. Rosemblatt, C. Hidalgo, C. Vergara, N. Ikemoto, Immunological and biochemical properties of transverse tubule membranes isolated from rabbit skeletal muscle. Journal of Biological Chemistry. ,vol. 256, pp. 8140- 8148 ,(1981) , 10.1016/S0021-9258(18)43399-6
A. Lewis Farr, Oliver H. Lowry, Rose J. Randall, Nira J. Rosebrough, Protein Measurement with the Folin Phenol Reagent Journal of Biological Chemistry. ,vol. 193, pp. 265- 275 ,(1951)
Jean-Pierre GALIZZI, Michel FOSSET, Michel LAZDUNSKI, Properties of receptors for the Ca2+-channel blocker verapamil in transverse-tubule membranes of skeletal muscle. Stereospecificity, effect of Ca2+ and other inorganic cations, evidence for two categories of sites and effect of nucleoside triphosphates. FEBS Journal. ,vol. 144, pp. 211- 215 ,(1984) , 10.1111/J.1432-1033.1984.TB08451.X