Neuronal distribution and functional characterization of the calcium channel α2δ‐2 subunit

摘要: The auxiliary calcium channel alpha2delta subunit comprises a family of three genes, alpha2delta-1 to 3, which are expressed in tissue-specific manner. alpha2delta-2 mRNA is found the heart, skeletal muscle, brain, kidney, liver and pancreas. We report here for first time identification functional characterization splice variants their distribution mouse brain. differ alpha2 delta protein by eight amino acid residues, respectively, differentially cardiac tissue human medullary thyroid carcinoma (hMTC) cells. In situ hybridization brain sections revealed highest expression Purkinje cell layer cerebellum, habenulae septal nuclei, lower cerebral cortex, olfactory bulb, thalamic hypothalamic as well inferior superior colliculus. As data did not suggest specific colocalization with any alpha1 subunit, coexpression studies were carried out either high-voltage-gated channels, alpha1C, alpha1E or alpha1A, low-voltage-gated channel, alpha1G. Coexpression increased current density, shifted voltage dependence activation inactivation alpha1A subunits hyperpolarizing direction, accelerated decay steady-state alpha1G current.