Androgenetic Embryonic Stem Cells Form Neural Progenitor Cells In Vivo and In Vitro

摘要: Uniparental zygotes with two paternal (androgenetic [AG]) or maternal (gynogenetic [GG]; parthenogenetic [PG]) genomes are not able to develop into viable offspring but can form blastocysts from which embryonic stem cells (ESCs) be derived. Although some aspects of the in vitro and vivo differentiation potential PG GG ESCs several species have been studied, developmental capacity AG is much less clear. Here, we investigate murine undergo neural differentiation. We observed that differentiate pan-neural progenitor (pnPCs) further give rise express neuronal- astroglial-specific markers. Neural progeny vitro-differentiated exhibited fidelity expression six imprinted genes analyzed, exception Ube3a. Bisulfite sequencing for imprinting control regions suggested pnPCs predominantly maintained their methylation pattern. Following blastocyst injection biparental (normal fertilized [N]) ESCs, found widespread evenly distributed contribution ESC-derived both N chimeric early fetal brains. isolated brains by fluorescence-activated cell sorting similar neurosphere-initiating frequencies multilineage potential. Our results indicate progenitor/stem do differ self-renewal Disclosure conflicts interest at end this article.