Production of citric acid by immobilized Aspergillus niger using a rotating biological contactor (RBC)

作者: Wang Jianlong

DOI: 10.1016/S0960-8524(00)00053-5

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摘要: Abstract Production of citric acid by Aspergillus niger was studied in a rotating biological contactor (RBC) consisting plastic disks mounted on horizontal shaft with polyurethane foam (PUF), as porous biomass support, attached each side the RBC disks. Mycelia A. formed biofilm immobilized cells surface PUF. The RBC–PUF system operated rotational speed 10 rev/min during stage formation. mature exposed to fermentation medium and air space alternately. results showed that volumetric productivity obtained (0.896 g/l h) almost three times higher than stirred-tank fermenter (0.33 h). active for over 8-cycle periods production repetitive use without loss bioactivity.

参考文章(12)
Yashoda Mittal, I. M. Mishra, B. S. Varshney, Characterization of metabolically active developmental stage of Aspergillus niger cells immobilized in polyacrylamide gel Biotechnology Letters. ,vol. 15, pp. 41- 46 ,(1993) , 10.1007/BF00131550
J.R. Marier, M. Boulet, Direct determination of citric acid in milk with an improved pyridine-acetic anhydride method. Journal of Dairy Science. ,vol. 41, pp. 1683- 1692 ,(1958) , 10.3168/JDS.S0022-0302(58)91152-4
H. Eikmeier, H.J. Rehm, Production of citric acid with immobilized Aspergillus niger Applied Microbiology and Biotechnology. ,vol. 20, pp. 365- 370 ,(1984) , 10.1007/BF00261936
A. Sanrom�n, J. Pintado, J. M. Lema, A comparison of two techniques (adsorption and entrapment) for the immobilization of Aspergillus niger in polyurethane foam Biotechnology Techniques. ,vol. 8, pp. 389- 394 ,(1994) , 10.1007/BF00154309
G. L. Miller, Use of Dinitrosalicylic Acid Reagent for Determination of Reducing Sugar Analytical Chemistry. ,vol. 31, pp. 426- 428 ,(1959) , 10.1021/AC60147A030
YongHee Lee, ChangWoo Lee, HoNam Chang, Citric acid production by Aspergillus niger immobilized on polyurethane foam Applied Microbiology and Biotechnology. ,vol. 30, pp. 141- 143 ,(1989) , 10.1007/BF00264001
KUSH GARG, AND CHANDRA B. SHARMA, CONTINUOUS PRODUCTION OF CITRIC ACID BY IMMOBILIZED WHOLE CELLS OF ASPERGILLUS NIGER Journal of General and Applied Microbiology. ,vol. 38, pp. 605- 615 ,(1992) , 10.2323/JGAM.38.605
R.D. Tyagi, F.T. Tran, A.K.M.M. Chowdhury, A pilot study of biodegradation of petroleum refinery wastewater in a polyurethane-attached RBC Process Biochemistry. ,vol. 28, pp. 75- 82 ,(1993) , 10.1016/0032-9592(93)80010-E
J L Wang, P Liu, Comparison of citric acid production byAspergillus niger immobilized in gels and cryogels of polyacrylamide Journal of Industrial Microbiology & Biotechnology. ,vol. 16, pp. 351- 353 ,(1996) , 10.1007/BF01570114
Ningjun Cao, Jianxin Du, Cheeshan Chen, Cheng S. Gong, George T. Tsao, Production of fumaric acid by immobilized rhizopus using rotary biofilm contactor. Applied Biochemistry and Biotechnology. ,vol. 63, pp. 387- 394 ,(1997) , 10.1007/BF02920440