Determination of plasmid DNA concentration maintained by nonculturable Escherichia coli in marine microcosms.
作者： J J Byrd , J G Leahy , R R Colwell
DOI: 10.1128/AEM.58.7.2266-2270.1992
关键词:
摘要: The concentration of plasmid pBR322 DNA in nonculturable Escherichia coli JM83 was measured to determine whether the changed during survival E. marine and estuarine water. containing placed both sterile seawater water analyzed for (i.e., culturability) maintenance. remained stable 28 days an artificial microcosm, even though nonculturability achieved within 7 days. incubated natural or did not reach 30 Under all three conditions, maintained at approximately initial concentration. Cloning into pUC8 alter ability maintain vector DNA, when culture state, but decreased with time microcosm. We conclude that is able while state which appears be dependent upon copy number and/or presence foreign DNA.
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