Stimulation of alkaline phosphatase activity in Ishikawa cells induced by various phytoestrogens and synthetic estrogens

摘要: Abstract Xenoestrogens, phytoestrogens and synthetic estrogens, are able to bind estrogen receptors, mimic estrogenic activities in a cell tissue specific manner. For the characterization of environmental estrogens mainly mammary derived yeast based models have been used. The aim this study was therefore assess selected natural compounds an endometrial model. We measured relative potency (genistein, daidzein, coumestrol, some naringenins), (bisphenol A, octylphenol, nonylphenol, o , p ′-DDT), mycoestrogen (zearalanone) as well extracts Cimicifuga racemosa on alkaline phosphatase (AlkP) activity adenocarcinoma line Ishikawa. used modified multiwell plate vitro bioassay estrogen-specific dose-dependent enhancement AlkP line. Estradiol, which induced at levels low 10 −8  M, positive control. Most studied showed clear effect. Compared vehicle control (ethanol) all phyto- mycoestrogens, stimulated 2–4-fold concentration −6  M. chemicals bisphenol A nonylphenol effect octylphenol −5 Effects ′-DTT could not be measured. ICI 182,780, pure receptor antagonist, significantly inhibited these effects. latter result demonstrated dependency process. In summary, most industrial tested, behaved agonists terms stimulation activity.