Comprehensive analysis of 19q12 amplicon in human gastric cancers.

摘要: Amplification at 19q12 has been observed in multiple tumor types, while cyclin E1 (CCNE1) considered to be the key oncogene within this amplicon. We have previously applied cDNA microarray analysis systematically characterize gene expression patterns of gastric and nontumor samples. identified a cluster five tightly coregulated genes all located chromosome 19q12, including CCNE1. found that is highly expressed tumors compared Array based comparative genomic hybridization real-time PCR was used define boundary amplicon region approximately 200 kb. Interestingly, we some cases amplification not associated with DNA copy number gain CCNE1, suggesting other may also important function during tumorigenesis. high statistically correlated cell proliferation signature. Using SAM software, set 577 whose levels positively cluster. GO term revealed genelist enriched involved cycle regulation proliferation. In conclusion, array combined provides new powerful tool identify clusters which regulated by aberrations. addition, our study indicates vivo.