Focusing Transgene Expression in Drosophila by Coupling Gal4 With a Novel Split-LexA Expression System
作者： Chun-Yuan Ting , Stephanie Gu , Sudha Guttikonda , Tzu-Yang Lin , Benjamin H. White
DOI: 10.1534/GENETICS.110.126193
关键词:
摘要: Here we report the development of a ternary version LexA::VP16/LexAop system in which DNA-binding and trans-activating moieties are independently targeted using distinct promoters to achieve highly restricted, intersectional expression patterns. This Split LexA can be concatenated with Gal4/upstream activating sequence refine patterns existing Gal4 lines minimal genetic manipulations.
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