Differential expression of matrix metalloproteinases in activated c-ras-Ha-transfected immortalized human keratinocytes.

摘要: Elevated expression of matrix metalloproteinases (MMPs), a family secreted proteinases that degrade components basement membranes and connective tissues, is strongly correlated with malignant in various human epithelial cancers cancer cell lines. We have tested whether elevated levels MMP are also associated progression cutaneous squamous carcinoma. Constitutive steady-state mRNA protein encoded by three genes (matrilysin, gelatinases A B) were compared unique vitro model skin carcinogenesis. This composed the parental immortalized non-tumorigenic keratinocyte line (HaCaT), activated c-Harvey-ras-oncogene transfected variants (A-4, I-7 II-4). Although clone A-4 non-tumorigenic, clones II-4 exhibit benign tumorigenic phenotypes, respectively, after subcutaneous injection into athymic nude mice. Northern blot, Western zymogram analyses revealed MMP-specific patterns expression. matrilysin was markedly increased cells HaCaT, or cells. Secreted promatrilysin distinctly HaCaT Gelatinase gelatinase each transfectant HaCaT. Both active inactive forms detected. B transcripts not detected, but an EDTA-inhibitable activity comigrating moderately enhanced both Because 92-kDa secretion cells, related to invasiveness this model, it concluded constitutive these two MMPs acquisition phenotype, before phenotype.