Endocytic pathway of recombinant murine tumor necrosis factor in L-929 cells.

摘要: The fate of TNF after binding to the surface L-929 cells was followed by using murine rTNF coupled colloidal gold as a probe. A time-course study electron microscopy performed. Our results confirm previous indications obtained from biochemical studies suggesting that is internalized this cell type. They further directly show internalization proceeds through classical receptor-mediated endocytosis pathway, i.e., via clathrin-coated structures and endosomes before accumulation in secondary lysosomes.