Ultrastructural and molecular identification of a Wolbachia endosymbiont in a spider, Nephila clavata

作者: Hyun Woo Oh , Mi Gwang Kim , Sang Woon Shin , Kyung Sook Bae , Young Joon Ahn

DOI: 10.1046/J.1365-2583.2000.00218.X

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摘要: Wolbachia-like bacteria were observed in the egg cells of golden orb-weaving spider, Nephila clavata, by means transmission electron microscopy. The exhibited typical morphology Wolbachia, including three enveloping membranes. Based on amplification and sequencing partial 16S rDNA ftsZ gene, identified as intracellular, transovarially inherited alpha-proteobacteria invertebrates. Phylogenetic analysis based gene sequences invariably indicated that intracellular from N. clavata belonged to group A which found only insects. Clustering Wolbachia with indicates probably transferred horizontally between insects spider.

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