Non-invasive tumor detection in small animals using novel functional Pluronic nanomicelles conjugated with anti-mesothelin antibody.
作者： Hong Ding , Ken-Tye Yong , Wing-Chueng Law , Indrajit Roy , Rui Hu
DOI: 10.1039/C1NR00001B
关键词:
摘要: In this study QDs were encapsulated in carboxylated PluronicF127 (F127COOH) triblock polymeric micelles and conjugated with anti-mesothelin antibody for the purpose of alleviating potential toxicity, enhancing stability improving targeting efficiency CdTe/ZnS quantum dots (QDs) tumors. The amphiphilic polymer F127COOH contains hydrophilic poly(ethylene oxide) (PEO) hydrophobic poly(propylene (PPO) units. After encapsulating into F127 (F127COOH-QD) micelles, particles antibodies to allow cancerous areas. size monodispersed spherical QD-containing was determined be ∼120 nm by dynamic light scattering (DLS). critical micelle concentration (CMC) estimated 4.7 × 10−7 M. an vitro study, anti-methoselin (Me-F127COOH-QD) nanomicelles showed negligible cytotoxicity pancreatic cancer cells (Panc-1). Confocal microscopy demonstrated that Me-F127COOH-QD taken up more efficiently Panc-1 cells, due mediated targeting. An vivo imaging accumulated at tumor site 15 min after intravenous injection. addition, low toxicity nanomicellar formulation evaluated pathological assays. These results suggest anti-mesothein may serve as a promising nanoscale platform early human detection targeted drug delivery.
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