摘要: It is hardly an exaggeration to state that the introduction of radioimmunoassay (RIA) more than 25 years ago has been single most important methodological advance in endocrinology past 50 years. The ability measure notoriously small amounts hormone blood samples decisively shaped our current concepts regarding endocrine mechanisms, especially vertebrates. impact this technique on invertebrate a recent date. ecdysone RIA 16 (Borst and O’Connor, 1972; see Chapter 6) had enormous consequences for development arthropod endocrinology.