Use of Large-Scale Two-Dimensional ISODALT Gel Electrophoresis System in Immunology

摘要: Publisher Summary This chapter focuses on the usage of large-scale two-dimensional ISODALT gel electrophoresis system in immunology. At Argonne National Laboratory, system, where ISO describes charge separation and DALT stands for size separation, is a large, multipurpose operation. The also one version adapted at Basel Institute Immunology specific problems occurring In 1975, O’Farrell developed an analytical technique resolution complex protein mixture using combination two procedures—charge separation. electrophoretograms obtained by this allow fine comparison pattern within single run. final aim to perform computer, function which not only compare gels adjusted matching, stretching, fitting but analyze comparing presence or absence individual spots constellations with cumulative experimental data file.