Changes in the protein pattern of murine B cells after mitogenic stimulation.
作者： Jack Kettman , Ivan Lefkovits
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摘要: The technique of two-dimensional gel electrophoresis has been applied to study the changes in protein patterns occurring murine B lymphocytes after activation with synergistic cell mitogens lipopolysaccharide and dextran sulfate. Whole lysates from [35S]methionine-labeled mitogen-activated cells were analyzed radiofluorographs gels compared. We found that a large number proteins appear disappear during 5-day period (74/206 spots nonimmunoglobulin region gel). pattern (in region) was also compared T cells. identified 19 cell-specific 29 proteins, remaining 1000 or so being common.
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sci-hub.se 参考文章(13)
Gayle D. Wetzel, John R. Kettman, Activation of murine B cells Cellular Immunology. ,vol. 61, pp. 176- 189 ,(1981) , 10.1016/0008-8749(81)90364-6
G D Wetzel, J R Kettman, Activation of murine B lymphocytes. III. Stimulation of B lymphocyte clonal growth with lipopolysaccharide and dextran sulfate. Journal of Immunology. ,vol. 126, pp. 723- 728 ,(1981)
R P Tracy, R M Currie, R A Kyle, D S Young, Two-dimensional gel electrophoresis of serum specimens from patients with monoclonal gammopathies. Clinical Chemistry. ,vol. 28, pp. 900- 907 ,(1982) , 10.1093/CLINCHEM/28.4.900
R Duncan, E H McConkey, How many proteins are there in a typical mammalian cell Clinical Chemistry. ,vol. 28, pp. 749- 755 ,(1982) , 10.1093/CLINCHEM/28.4.749
Jan Andersson, Antonio Coutinho, Waldemar Lernhardt, Fritz Melchers, Clonal growth and maturation to immunoglobulin secretion in vitro of every growth-inducible B lymphocyte Cell. ,vol. 10, pp. 27- 34 ,(1977) , 10.1016/0092-8674(77)90136-2
Dorothy Yuan, Ellen S. Vitetta, Structural studies of cell surface and secreted IgG in LPS-stimulated murine B cells Molecular Immunology. ,vol. 20, pp. 367- 375 ,(1983) , 10.1016/0161-5890(83)90018-4
Jack Kettman, Ivan Lefkovits, An attempt to assess the overall diversity of murine T cells using two‐dimensional gel electrophoresis European Journal of Immunology. ,vol. 14, pp. 769- 777 ,(1984) , 10.1002/EJI.1830140902
F. Melchers, J. Andersson, IgM in bone marrow-derived lymphocytes. Changes in synthesis, turnover and secretion, and in numbers of molecules on the surface of B cells after mitogenic stimulation European Journal of Immunology. ,vol. 4, pp. 181- 188 ,(1974) , 10.1002/EJI.1830040306
Charles Sidman, B lymphocyte differentiation and the control of IgM μ chain expression Cell. ,vol. 23, pp. 379- 389 ,(1981) , 10.1016/0092-8674(81)90133-1
J. Kettman, A. Soederberg, I. Lefkovits, Heterogeneity of B cell clones: Immunoglobulin-secreting subsets Cellular Immunology. ,vol. 66, pp. 70- 77 ,(1982) , 10.1016/0008-8749(82)90158-7