Adaptive gene profiling of Mycobacterium tuberculosis during sub-lethal kanamycin exposure

摘要: Resistance to anti-tuberculosis drugs is a formidable obstacle effective tuberculosis (TB) treatment and prevention globally. New forms of multidrug, extensive drug total resistance Mycobacterium (Mtb) causing serious threat human as well animal's population. Mtb shows diverse adaptability under stress conditions especially antibiotic treatment, however underlying physiological mechanism remained elusive. In present study, we investigated Mtb's response adaptation with reference gene expression during sub-lethal kanamycin exposure. were cultured control conditions, where half sub-cultured every 3-days observe serial same the remaining subjected RNA-seq. We identified 98 up-regulated 198 down-regulated responsive genes compared through differential analysis, which Ra1750 Ra3160 most genes. adaptive found Ra1750, Ra3160, Ra3161, Ra3893 Ra2492 up-regulation at early stage gradually showed low levels later stages The Ra3161 further confirmed by real time qPCR. These results suggested that these contributed in Our findings may aid edify potential targets for development against tuberculosis.