Rv3133c/dosR is a transcription factor that mediates the hypoxic response of Mycobacterium tuberculosis.

摘要: Unlike many pathogens that are overtly harmful to their hosts, Mycobacterium tuberculosis can persist for years within humans in a clinically latent state. Latency is often linked hypoxic conditions the host. Among M. genes induced by hypoxia putative transcription factor, Rv3133c/DosR. We performed targeted disruption of this locus followed transcriptome analysis wild-type and mutant bacilli. Nearly all powerfully regulated require Rv3133c/DosR induction. Computer identified consensus motif, variant which located upstream nearly rapidly hypoxia. Further, binds two copies motif response gene alpha-crystallin. Mutations binding sites abolish both as well induction downstream reporter gene. Also, mutation experiments with confirmed sequence-based predictions C-terminus responsible DNA aspartate at position 54 essential function. Together, these results demonstrate factor two-component regulator class, it primary mediator signal tuberculosis.