Dietary omega-3 polyunsaturated fatty acids alter the fatty acid composition of hepatic and plasma bioactive lipids in C57BL/6 mice: a lipidomic approach.

摘要: Background Omega (n)-3 polyunsaturated fatty acids (PUFA) are converted to bioactive lipid components that important mediators in metabolic and physiological pathways; however, which compounds metabolically active, their mechanisms of action still not clear. We investigated using lipidomic techniques, the effects diets high n-3 PUFA on acid composition various lipids plasma liver. Methodology Principal Findings Female C57BL/6 mice were fed semi-purified (20% w/w fat) containing varying amounts before mating, during gestation lactation, until weaning. Male offspring continued mothers’ for 16 weeks. Hepatic extracted presence non-naturally occurring internal standards, tandem electrospray ionization mass spectrometry methods used measure acyl compositions. There was no significant difference total concentrations phospholipids both groups. However, there a significantly higher concentration eicosapentaenoic phosphatidylcholine (PC), lysophosphatidylcholine (LPC), cholesteryl esters (CE) (p < 0.01) group compared low liver plasma. Plasma from also had free 0.05). differences different species phosphatidylethanolamine, triglycerides, sphingomyelin ceramides. Conclusions/Significance Our findings reveal first time diet caused enrichment PC, LPC, CE mice. unesterified lipids, thus altering will have roles.