PNGase F-mediated incorporation of 18O into glycans for relative glycan quantitation
作者： Wei Zhang , Weiqian Cao , Jiangming Huang , Hong Wang , Ji Wang
DOI: 10.1039/C4AN02073A
关键词:
摘要: PNGase F-catalyzed glycosylation site 18O-labeling is a widely used method for glycoprotein quantitation owing to its efficiency and simplicity. However, glycan 18O-labeling, which offers advantages glycomics, has not yet been developed. In this study, F-mediated incorporation of 18O into glycans during the N-glycan release from glycoproteins by F was finally realized, named as PCGOL (PNGase 18O-labeling), potential strategy relative quantitation. This new showed good linearity high reproducibility within at least 2 orders magnitude in dynamic range. Furthermore, combined with our previously developed TOSIL (tandem stable isotope labeling N-glycoproteome quantitation) can be comprehensive N-glycosylation quantification, achieving simultaneous quantification glycans, glycopeptides single workflow, also analyze changes immunoglobulin G (IgG) associated hepatocellular carcinoma present work.
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