Lectin affinity capture, isotope-coded tagging and mass spectrometry to identify N-linked glycoproteins
作者： Hiroyuki Kaji , Haruna Saito , Yoshio Yamauchi , Takashi Shinkawa , Masato Taoka
DOI: 10.1038/NBT829
关键词:
摘要: … is captured by lectin-affinity chromatography. Next, N-linked glycosylation sites are labeled with 18 O … in our initial study without the isotope tag, occurred before the PNGase F digestion. …
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