Parameters affecting reproducibility in capillary electrophoresis.

摘要: It is well known that poor quantitative reproducibility substantially limits the practical implementation of capillary electrophoresis (CE) separations in chemical analysis. The principal sources variance observed peak areas are irreproducible flow rate, which influences on-column detector response, and inconsistent injection volume or amount. An overview studies by researchers to address issue will be presented. In addition, current efforts our laboratory assess for dansylated amino acids using an automated CE system presented related when appropriate body existing knowledge on this important topic. A comparison different methods (hydrostatic vs. electrokinetic) approaches (e.g., high low pressure), effect random changes electroosmotic (EOF) due air bubbles capillary, choice certain integration parameters terms area Under optimum conditions relative standard deviation (RSD) values raw typically 2.0%. With nonoptimum with capillary), RSD can degrade. However, normalizing retention times, internal standards, electrophoretic produces a range 1.4-2.3%.