Source of oncofetal ED-B-containing fibronectin: implications of production by both tumor and endothelial cells.

摘要: ED-B fibronectin (FN) is a FN isoform derived from alternative splicing of the primary transcript single gene. Its expression on tumor stroma and neoformed vasculature its absence, with few exceptions, in normal adult tissues imply prognostic diagnostic value for FN. We investigated location source because this will be importance both understanding role development designing strategies to target molecule. have confirmed that expressed majority breast colorectal carcinoma tissue samples, strong immunohistochemical staining around cells stroma. No neovasculature was seen. produced by range endothelial (both transformed) cell lines, as detected reverse transcription-PCR, but not at plasma membrane. Strong human seen xenografts. These data indicate neoplastic can act tumors. The lack surface lines has clear implications design therapeutic which