A simplified protocol for fluorescence in situ hybridization with repetitive DNA probes and its use in clinical cytogenetics
作者： O. Bartsch , E. Schwinger
DOI: 10.1111/J.1399-0004.1991.TB03067.X
关键词:
摘要: A method for chromosome-specific staining and its use in clinical cytogenetics is described. This fluorescence situ hybridization protocol repetitive DNA probes results yellow-green fluorescent signals on orange-red stained chromosomes. Special characteristics are simplicity, the of digoxigenin-11-dUTP labeling, combination high stringency criteria low washing. The particularly advantageous cases with structurally abnormal extra chromosomes (ESACs), marker gonosomal origin, chromosomal mosaicism. It may also facilitate screening fragile X. can be done within 1 working-day.
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