Biomonitoring of the genotoxic potential of aqueous extracts of soils and bottom ash resulting from municipal solid waste incineration, using the comet and micronucleus tests on amphibian (Xenopus laevis) larvae and bacterial assays (Mutatox and Ames tests).
作者： F MOUCHET , L GAUTHIER , C MAILHES , M JOURDAIN , V FERRIER
DOI: 10.1016/J.SCITOTENV.2005.02.031
关键词:
摘要: The management of contaminated soils and wastes is a matter considerable human concern. present study evaluates the genotoxic potential aqueous extracts two (leachates) bottom ash resulting from municipal solid waste incineration (MSWIBA percolate), using amphibian larvae (Xenopus laevis). Soil A was by residues solvents metals B polycyclic aromatic hydrocarbons metals. MSWIBA predominantly Two endpoints were analysed in circulating erythrocytes taken larvae: clastogenic and/or aneugenic effects (micronucleus induction) after 12 days exposure DNA-strand-breaking potency (comet assay) 1 exposure. In addition, vitro bacterial assays (Mutatox Ames tests) carried out results compared with those test. Physicochemical analyses also into account. Results obtained amphibians established genotoxicity comet assay revealed that they first day latter test could thus be considered as genotoxicity-screening tool. Although persisted days' exposure, DNA damage decreased overall between percolate, contrast to soil leachates. Bacterial tests detected only for leachate (Mutatox). confirm ecotoxicological relevance model underscore importance bioassays, complement physico-chemical data, risk evaluation.
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