Chitosan/interfering RNA nanoparticle mediated gene silencing in disease vector mosquito larvae.

摘要: Vector mosquitoes inflict more human suffering than any other organism-and kill one million people each year. The mosquito genome projects facilitated research in new facets of biology, including functional genetic studies the primary African malaria vector Anopheles gambiae and dengue yellow fever Aedes aegypti. RNA interference- (RNAi-) mediated gene silencing has been used to target genes interest both these disease species. Here, we describe a procedure for preparation chitosan/interfering nanoparticles that are combined with food ingested by larvae. This technically straightforward, high-throughput, relatively inexpensive methodology, which is compatible long double stranded (dsRNA) or small interfering (siRNA) molecules, successful knockdown number different A. aegypti Following larval feedings, knockdown, verified through qRT-PCR situ hybridization, can persist at least late pupal stage. methodology may be applicable wide variety insect species, agricultural pests, as well non-model organisms. In addition its utility laboratory, future, chitosan, an inexpensive, non-toxic biodegradable polymer, could potentially utilized field.