Assessing the enzymatic effects of cellulases and LPMO in improving mechanical fibrillation of cotton linters

摘要: The increasing interest in replacing petroleum-based products by more sustainable materials the packaging sector gives relevance to cellulose as a biodegradable natural resource. Moreover, its properties can be modified physically, chemically or biotechnologically order obtain new bioproducts. Refined cotton linters with high content were treated hydrolytic (cellulases) and oxidative (LPMO Laccase_Tempo) enzymes evaluate their effect on fibre improving mechanical fibrillation. Cellulases released cellooligosaccharides, reducing length partially degrading cellulose. They also improved fibrillation yielding up 18% of nanofibrillated (NFC). LPMO introduced slight amount COOH groups fibres, releasing cellobionic acid effluents. action cellulases was after treatment; however, created disappeared from fibres. After LPMO–cellulase-treated 23% yield NFC obtained. Laccase_Tempo treatment fibres cotton, 10% NFC. Degree polymerization reduced Laccase_Tempo, while did not significantly affect it but produced higher reduction length. combined cellulase provided films transparency (86%), crystallinity (92%), smoothness barrier air water than casted non-treated commercial enzymatic boosted linters, production providing bioproducts properties.