C ux2 activity defines a subpopulation of perinatal neurogenic progenitors in the hippocampus

摘要: The hippocampus arises from the medial region of subventricular (SVZ) within telencephalon. It is one two regions in postnatal brain that harbors neural progenitors (NPs) capable giving rise to new neurons. Neurogenesis restricted subgranular zone (SGZ) dentate gyrus (DG) where it contributes generation granule cell layer (gcl) thought SGZ are heterogeneous, differing their morphology, expression profiles, and developmental potential, however currently unknown whether they display differences origins fate-restriction DG. Here we demonstrate Cux2 a marker for nascent neurons perinatal brain. was expressed presumptive hippocampal forming embryonic forebrain E14.5 onwards. At fetal stages, early-forming Prox1(+) as well SVZ DG germinal matrix. In brain, several types DG, including Nestin/Sox2 double-positive radial glia, Sox2(+) cells lacked glial process, DCX(+) neuroblasts, Calretinin-expressing Another domain characterized by low level emerged Calbindin(+) developing blades. We used Cux2-Cre mice genetic fate-mapping studies showed almost exclusive labeling Calbindin-positive gcl neurons, but not any progenitor or astroglia. This suggests Cux2(+) directly differentiate into do self-renew. Interestingly, profiling fate revealed an outside-in formation likely reflecting activity germinative matrices during maturation. However, morphogenesis proceeded largely normally hypomorphic mutants lacking expression. Taken together conclude reflects neurogenesis identifies non-self-renewing NPs SGZ.