Intragenic Homozygous Deletions of MTS1 Gene in Gastric Cancer in Taiwan
作者： Ming-Shiang Wu , Ya-Wen Lin , Jin-Chuan Sheu , Hsiu-Po Wang , Jin-Town Wang
DOI: 10.1111/J.1349-7006.1996.TB03109.X
关键词:
摘要: The multiple tumor suppressor 1 (MTS1) and 2 (MTS2) genes, located on chromosome 9p21, have been reported to be deleted or mutated in many malignant cell lines a high percentage of some primary carcinomas. To determine whether these genes are altered, if so, what is the nature alterations, human gastric adenocarcinoma, we investigated their frequency mutation by Southern blotting, polymerase chain reaction (PCR) direct sequencing 55 patients. Furthermore, loss heterozygosity (LOH) 9p21 at IFNA locus D9S171 was assessed. Homozygous deletions exon MTS1 gene were identified 5 (9.1%) tumors. No deletion MTS2 noted. LOH observed 7 (14.3%) 49 informative cases (5 locus, one case with combined homozygous MTS1). Direct PCR products did not reveal any point These data indicate that alterations infrequently encountered. Additional studies more micro-satellite markers near mandatory elucidate another exists vicinity adenocarcinoma.
sci-hub.se 参考文章(26)
G Reifenberger, K Ichimura, V P Collins, E E Schmidt, CDKN2 (p16/MTS1) gene deletion or CDK4 amplification occurs in the majority of glioblastomas. Cancer Research. ,vol. 54, pp. 6321- 6324 ,(1994)
Jin Jen, J Wade Harper, Sandra H Bigner, Darell D Bigner, Nickolas Papadopoulos, Sanford Markowitz, James KV Willson, Kenneth W Kinzler, Bert Vogelstein, None, Deletion of p16 and p15 Genes in Brain Tumors Cancer Research. ,vol. 54, pp. 6353- 6358 ,(1994)
Tetsuro Nishihira, Shozo Mori, Koh Miura, Yusuke Nakamura, Takahisa Aoki, Takahiro Mori, Frequent somatic mutation of the MTS1/CDK4I (multiple tumor suppressor/cyclin-dependent kinase 4 inhibitor) gene in esophageal squamous cell carcinoma. Cancer Research. ,vol. 54, pp. 3396- 3397 ,(1994)
Chia-Tung Shun, Jin-Chuan Sheu, Ming-Shiang Wu, Wei-Jei Lee, Teh-Hong Wang, Jaw-Town Lin, Occurrence of Microsatellite Instability in Gastric Carcinoma Is Associated with Enhanced Expression of erbB-2 Oncoprotein Cancer Research. ,vol. 55, pp. 1428- 1430 ,(1995)
Osuke Washimi, M Nagatake, Hirotaka Osada, Ryuzo Ueda, T Koshikawa, Tsuneo Seki, Toshitada Takahashi, Takashi Takahashi, In vivo occurrence of p16 (MTS1) and p15 (MTS2) alterations preferentially in non-small cell lung cancers Cancer Research. ,vol. 55, pp. 514- 517 ,(1995)
R. Day, B. Johnson, M. Skolnick, A Kamb, N. Gruis, J Weaver-Feldhaus, Q Liu, K Harshman, S. Tavtigian, E Stockert, A cell cycle regulator potentially involved in genesis of many tumor types Science. ,vol. 264, pp. 436- 440 ,(1994) , 10.1126/SCIENCE.8153634
Tony Hunter, Jonathan Pines, Cyclins and cancer II: Cyclin D and CDK inhibitors come of age Cell. ,vol. 79, pp. 573- 582 ,(1994) , 10.1016/0092-8674(94)90543-6
Tsutomu Nobori, Kaoru Miura, David J. Wu, Augusto Lois, Kenji Takabayashi, Dennis A. Carson, Deletions of the cyclin-dependent kinase-4 inhibitor gene in multiple human cancers Nature. ,vol. 368, pp. 753- 756 ,(1994) , 10.1038/368753A0
N. Hayashi, Y. Sugimoto, E. Tsuchiya, M. Ogawa, Y. Nakamura, Somatic Mutations of the MTS (Multiple Tumor Suppressor) 1/CDK41 (Cyclin-Dependent Kinase-4 Inhibitor) Gene in Human Primary Non-small Cell Lung Carcinomas Biochemical and Biophysical Research Communications. ,vol. 202, pp. 1426- 1430 ,(1994) , 10.1006/BBRC.1994.2090
Eiichi Tahara, Genetic alterations in human gastrointestinal cancers. The application to molecular diagnosis Cancer. ,vol. 75, pp. 1410- 1417 ,(1995) , 10.1002/1097-0142(19950315)75:6+<1410::AID-CNCR2820751504>3.0.CO;2-O