The transcription factor PRO44 and the histone chaperone ASF1 regulate distinct aspects of multicellular development in the filamentous fungus Sordaria macrospora

摘要: Fungal fruiting bodies are complex three-dimensional structures that formed to protect and disperse the sexual spores. Their morphogenesis requires concerted action of numerous genes; however, at molecular level, spatio-temporal sequence events leading mature body is largely unknown. In previous studies, transcription factor gene pro44 histone chaperone asf1 were shown be essential for formation in ascomycete Sordaria macrospora. Both PRO44 ASF1 predicted act on regulation expression nucleus, mutants both genes blocked same stage development. Thus, we hypothesized might involved similar aspects transcriptional regulation. this study, characterized their roles development more detail. The protein forms homodimers, localizes strongly expressed outer layers developing young body. Analysis single double three other chromatin modifier genes, cac2, crc1, rtt106, showed only whereas cac2 rtt106 have redundant functions process. RNA-seq analysis revealed distinct development, with acting as a suppressor weakly during morphogenesis. This most likely not due global mislocalization nucleosomes micrococcal nuclease-sequencing did reveal differences nucleosome spacing positioning around start sites between Δasf1 wild type. However, bisulfite sequencing decrease DNA methylation Δasf1, which reason observed changes expression. Transcriptome required correct extracellular metabolism. Deletion putative asm2, downregulated Δpro44, results defects ascospore maturation. summary, indicate fungi.