Fibroblasts potentiate blood vessel formation partially through secreted factor TIMP-1.
作者： Hua Liu , Bo Chen , Brenda Lilly
DOI: 10.1007/S10456-008-9102-8
关键词:
摘要: During wound repair, new blood vessels form in response to angiogenic signals emanating from injured tissues. Dermal fibroblasts are known play an important role healing, and have been linked angiogenesis; therefore, we sought understand the mechanisms through which these cells control vessel formation. Using a three-dimensional angiogenesis assay demonstrate that dermal enhance tube-forming potential of endothelial cells, this augmentation is partially due secreted factors present conditioned media. Interestingly, identified tissue inhibitor metalloproteinase-1 (TIMP-1) as factor uniquely by fibroblasts, addition exogenous TIMP-1 increased assembly. The enhancing activity was matrix metalloproteinase (MMP)-dependent, since mutant version unable promote angiogenesis. Consistent with this, chemical inhibition MMP-2/9 showed similar increase angiogenesis, MMP-9 blocked effect TIMP-1. We further demonstrated inhibits production tumstatin, anti-angiogenic fragment collagen IV produced cleavage. Our results support notion regulate formation multiple mediators, provide novel evidence fibroblast-derived acts on pro-angiogenic capacity.
springer.com
utsystem.edu参考文章(41)
Ed Harlow, David Lane, Using Antibodies: A Laboratory Manual ,(1998)
Diane C. Darland, Patricia A. D'Amore, Cell-cell interactions in vascular development. Current Topics in Developmental Biology. ,vol. 52, pp. 107- 149 ,(2001) , 10.1016/S0070-2153(01)52010-4
Gabriele Bergers, Rolf Brekken, Gerald McMahon, Thiennu H. Vu, Takeshi Itoh, Kazuhiko Tamaki, Kazuhiko Tanzawa, Philip Thorpe, Shigeyoshi Itohara, Zena Werb, Douglas Hanahan, Matrix metalloproteinase-9 triggers the angiogenic switch during carcinogenesis Nature Cell Biology. ,vol. 2, pp. 737- 744 ,(2000) , 10.1038/35036374
May J Reed, Teruhiko Koike, Eman Sadoun, E Helene Sage, Pauli Puolakkainen, None, Inhibition of TIMP1 enhances angiogenesis in vivo and cell migration in vitro. Microvascular Research. ,vol. 65, pp. 9- 17 ,(2003) , 10.1016/S0026-2862(02)00026-2
Momomi Saito, Maho Hamasaki, Masabumi Shibuya, Induction of tube formation by angiopoietin-1 in endothelial cell/fibroblast co-culture is dependent on endogenous VEGF. Cancer Science. ,vol. 94, pp. 782- 790 ,(2003) , 10.1111/J.1349-7006.2003.TB01519.X
Wen-Hui Zhu, Xiaodu Guo, Sergio Villaschi, Roberto Francesco Nicosia, Regulation of Vascular Growth and Regression by Matrix Metalloproteinases in the Rat Aorta Model of Angiogenesis Laboratory Investigation. ,vol. 80, pp. 545- 555 ,(2000) , 10.1038/LABINVEST.3780060
D. W. Powell, Water transport revisited The Journal of Physiology. ,vol. 514, pp. 1- 1 ,(1999) , 10.1111/J.1469-7793.1999.001AF.X
Martin N. Nakatsu, Richard C.A. Sainson, Jason N. Aoto, Kevin L. Taylor, Mark Aitkenhead, Sofía Pérez-del-Pulgar, Philip M. Carpenter, Christopher C.W. Hughes, Angiogenic sprouting and capillary lumen formation modeled by human umbilical vein endothelial cells (HUVEC) in fibrin gels: the role of fibroblasts and Angiopoietin-1. Microvascular Research. ,vol. 66, pp. 102- 112 ,(2003) , 10.1016/S0026-2862(03)00045-1
Gregory Bix, Renato V. Iozzo, Matrix revolutions: ‘tails’ of basement-membrane components with angiostatic functions Trends in Cell Biology. ,vol. 15, pp. 52- 60 ,(2005) , 10.1016/J.TCB.2004.11.008
D.C Darland, L.J Massingham, S.R Smith, E Piek, M Saint-Geniez, P.A D'Amore, Pericyte production of cell-associated VEGF is differentiation-dependent and is associated with endothelial survival. Developmental Biology. ,vol. 264, pp. 275- 288 ,(2003) , 10.1016/J.YDBIO.2003.08.015