Immunohistochemical Localization of Xanthine Oxidase in Human Tissues.
作者： Yuji Moriwaki , Tetsuya Yamamoto , Kei Yamaguchi , Michio Suda , Jun-ichi Yamakita
DOI: 10.1267/AHC.29.153
关键词:
摘要: We evaluated the immunohistochemical localization of xanthine oxidase in various human tissues. Xanthine was purified from cadaver liver. Polyclonal antibody against raised a rabbit. Immunoblot analysis showed that reacted specifically with one band whose position corresponded enzyme. Immunostaining paraffin-embedded tissue sections intense reactivity following tissues: surface epithelium tongue, esophagus, and trachea, sweat glands, mammary glands. Weak, but positive, observed other tissues, such as glandular cells small large intestine renal tubules, skeletal muscle, gastric epithelial cells, alveoli lung, spleen, liver cytoplasm. staining infiltrating lymphocytes (probably T-lymphocytes not B-lymphocytes) inflammatory lesions intestine. Its ubiquitous suggests is involved cell proliferation/differentiation, defense mechanisms, pathogenesis reperfusion injury.
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