Expression in Escherichia coli and sequencing of the coding region for the capsid protein of Dutch maedi-visna virus strain ZZV 1050: application of recombinant protein in enzyme-linked immunosorbent assay for the detection of caprine and ovine lentiviruses.

摘要: Maedi-visna in sheep and caprine arthritis-encephalitis goats are caused by two closely related widespread lentiviruses. The infections characterized life-long virus persistence slow induction of antiviral antibodies. diagnosis is based on the detection We have used polymerase chain reaction (PCR) to amplify a part gag gene coding for entire capsid protein parts matrix nucleocapsid proteins. Sequencing PCR fragment Dutch maedi-visna strain ZZV 1050 revealed 85 92% homology DNA deduced amino acid sequences, respectively, distantly Icelandic visna 1514. respective homologies with CO were 76 80%. was cloned into pGEX-2T expressed as glutathione S-transferase fusion protein. recombinant could be detected immunoblots using monoclonal antibody polyclonal antisera further purified glutathione-based affinity chromatography. Enzyme-linked immunosorbent assay shown sensitive specific diagnostic tool lentiviral infection sheep.