Theoretical and practical aspects of recombinant DNA techniques relevant to the clinical sciences

摘要: In the past decade recombinant DNA technology has expanded out from academic environment and into industrial clinical laboratories around world. Vaccines diagnostic kits based on DNA-derived products are now available with many more practical applications soon to follow. For such be used effectively it is important that potential limitations of new technologies widely understood by whole range specialists working in laboratory medicine. A review principal techniques genetic engineering cloning characterizing genes expression foreign antigens inEscherichia coli, yeast mammalian cells will presented. Some ways which these approaches have a major impact diagnosis treatment infections inherited metabolic disorders discussed.