[34] Maximizing gene expression on a plasmid using recombination in Vitro
作者： Thomas M. Roberts , Gail D. Lauer
DOI: 10.1016/0076-6879(79)68036-9
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摘要: Publisher Summary This chapter discusses a method for maximizing gene expression on plasmid using recombination in vitro . The amount of product produced transformed cells by plasmids carrying given promoter fusion is dependent the separation between and gene. describes method, utilizing combination restriction endonuclease cleavage digestion with Escherichia coli ( E. ) exonuclease III (Exo III) S1 nuclease, which allows to position fragment bearing lac Z region coding SD sequence at virtually any distance front cloned technique has been used produce strains, can provide up 200,000 monomers cro protein per cell. It useful constructing that direct production large amounts various proteins from other prokaryotic even eukaryotic cells.
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