Haplotype-Contained PCR Products Analysis by Sequencing with Selective Restriction of Primer Extension.
作者： Liu Wang , Pengfeng Xiao
DOI: 10.1155/2017/1397902
关键词:
摘要: We develop a strategy for haplotype analysis of PCR products that contained two adjacent heterozygous loci using sequencing with specific primers, allele-specific and ddNTP-blocked primers. To validate its feasibility, sets products, including SNPs, UGT1A1⁎6 (rs4148323) UGT1A1⁎28 (rs8175347), K1637K (rs11176013) S1647T (rs11564148), were analyzed. Haplotypes UGT1A1⁎28, successfully analyzed by Sanger Also, haplotypes S1647T, could not be determined primers but pyrosequencing As result, this method is able to effectively products. It simple, fast, irrespective short read length pyrosequencing. Overall, we fully hope it will provide new promising technology identify conventional in clinical samples.
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Chonlaphat Sukasem, Chalirmporn Atasilp, Pichai Chansriwong, Montri Chamnanphon, Apichaya Puangpetch, Ekapob Sirachainan, Development of Pyrosequencing Method for Detection of UGT1A1 Polymorphisms in Thai Colorectal Cancers. Journal of Clinical Laboratory Analysis. ,vol. 30, pp. 84- 89 ,(2016) , 10.1002/JCLA.21820
RYOUICHI TSUNEDOMI, SHOICHI HAZAMA, YUSUKE FUJITA, NAOKO OKAYAMA, SHINSUKE KANEKIYO, YUKA INOUE, SHIGEFUMI YOSHINO, TAKAHIRO YAMASAKI, YUTA KA SUEHIRO, KOJI OBA, HIDEYUKI MISHIMA, JUNICHI SAKAMOTO, YOSHIHIKO HAMAMOTO, MASAKI OKA, A novel system for predicting the toxicity of irinotecan based on statistical pattern recognition with UGT1A genotypes International Journal of Oncology. ,vol. 45, pp. 1381- 1390 ,(2014) , 10.3892/IJO.2014.2556
Philip Paul, Josh Apgar, Single-molecule dilution and multiple displacement amplification for molecular haplotyping. BioTechniques. ,vol. 38, pp. 553- 559 ,(2005) , 10.2144/05384ST01
Jonathan Marchini, David Cutler, Nick Patterson, Matthew Stephens, Eleazar Eskin, Eran Halperin, Shin Lin, Zhaohui S Qin, Heather M Munro, Gonçalo R Abecasis, Peter Donnelly, None, A Comparison of Phasing Algorithms for Trios and Unrelated Individuals The American Journal of Human Genetics. ,vol. 78, pp. 437- 450 ,(2006) , 10.1086/500808
Kristin G. Ardlie, Leonid Kruglyak, Mark Seielstad, Patterns of linkage disequilibrium in the human genome Nature Reviews Genetics. ,vol. 3, pp. 299- 309 ,(2002) , 10.1038/NRG777
Jer-Horng Wu, Pei-Ying Hong, Wen-Tso Liu, Quantitative effects of position and type of single mismatch on single base primer extension Journal of Microbiological Methods. ,vol. 77, pp. 267- 275 ,(2009) , 10.1016/J.MIMET.2009.03.001
Brett M. Ledeker, Susan K. De Long, The effect of multiple primer-template mismatches on quantitative PCR accuracy and development of a multi-primer set assay for accurate quantification of pcrA gene sequence variants Journal of Microbiological Methods. ,vol. 94, pp. 224- 231 ,(2013) , 10.1016/J.MIMET.2013.06.013
Christopher S. Carlson, Michael A. Eberle, Mark J. Rieder, Joshua D. Smith, Leonid Kruglyak, Deborah A. Nickerson, Additional SNPs and linkage-disequilibrium analyses are necessary for whole-genome association studies in humans. Nature Genetics. ,vol. 33, pp. 518- 521 ,(2003) , 10.1038/NG1128
Afshin Ahmadian, Maria Ehn, Sophia Hober, Pyrosequencing: History, biochemistry and future Clinica Chimica Acta. ,vol. 363, pp. 83- 94 ,(2006) , 10.1016/J.CCCN.2005.04.038
Genevieve Pont-Kingdon, Mohamed Jama, Christine Miller, Alison Millson, Elaine Lyon, Long-Range (17.7 kb) Allele-Specific Polymerase Chain Reaction Method for Direct Haplotyping of R117H and IVS-8 Mutations of the Cystic Fibrosis Transmembrane Regulator Gene The Journal of Molecular Diagnostics. ,vol. 6, pp. 264- 270 ,(2004) , 10.1016/S1525-1578(10)60520-X