Production and Characterization of Monoclonal and Polyclonal Antibodies to Forchlorfenuron
作者： Celia Suárez-Pantaleón , Josep V. Mercader , Consuelo Agulló , Antonio Abad-Somovilla , Antonio Abad-Fuentes
DOI: 10.1021/JF802261X
关键词:
摘要: The development of immunoassays for the detection plant growth regulator forchlorfenuron (CPPU) is described. To achieve that purpose, a set CPPU derivatives has been obtained by previous synthesis adequate p-aminophenyl alkanoic acid. Protein conjugates these compounds have used as immunogens to produce rabbit polyclonal antibodies and collection mouse monoclonal antibodies. Additionally, battery structural analogues target analyte synthesized characterization antibody binding. This strategy demonstrated most followed Landsteiner's principle, although some showing important deviations from this behavior also found. Finally, different assay formats developed with variety conjugates, rapid procedure optimized indirect ELISA format using In competitive ELISA, IC50 values below 0.5 nM were found LODs low 0.013 nM.
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